成果归属作者：

姚俊

成果归属机构：

水资源与环境学院

作者：

Liu, Houquan ; Yao, Jun ; Shi, Chunyu ; Duran, Robert ; Liu, Jianli ; Jiang, Shun ; Li, Miaomiao ; Pang, Wancheng ; Ma, Bo ; Cao, Ying ; Sunahara, Geoffrey

单位：

China Univ Geosci Beijing, Res Ctr Environm Sci & Engn, Sch Water Resource & Environm, MOE Key Lab Groundwater Circulat & Environm Evolut, 29 Xueyuan Rd, Beijing 100083, Peoples R China;Univ Pau & Pays lAdour, IPREM UMR CNRS 5254, E2S UPPA, BP 1155, F-64013 Pau, France;McGill Univ, Dept Nat Resource Sci, 21111 Lakeshore Dr, Ste Anne De Bellevue, PQ H9X 3V9, Canada

关键词：

ACID-MINE DRAINAGE; OXYGEN; COMMUNITY; CHROMIUM; IDENTIFICATION; REDUCTION; BACTERIAL; TOXICITY; NICHES; GROWTH

摘要：

Sulfate-reducing bacteria (SRB) are used in the remediation of mine pollution; however, the mechanism of stabilizing multiple heavy metal(loid)s by the SRB consortium under low oxygen conditions needs further study. Indigenous microflora were extracted from non-ferrous metal-contaminated soil co-inoculated with enriched SRB consortium and assembled as the HQ23 consortium. The presence of Desulfovibrio (SRB) in HQ23 was confirmed by 16S rRNA sequencing and qPCR. The effects of culture media, dissolved oxygen (DO), SO42- HQ23 growth rate, and the SO42--reducing activity were examined. Data indicates that the HQ23 sustained SRB function under low DO conditions (3.67 +/- 0.1 mg/L), but the SRB activity was inhibited at high DO content (5.75 +/- 0.39 mg/L). The HQ23 can grow from pH 5 to pH 9 and can decrease mobile or bioavailable Cr, Cu, and Zn concentrations in contaminated soil samples. FTIR revealed that Cu and Cr adsorbed to similar binding sites on bacteria, likely decreasing bacterial Cu toxicity. Increased abundances of DSV (marker for Desulfovibrio ) and nifH (N-fixation) genes were observed, as well as an accumulation of nitrate-N content in soils suggesting that HQ23 stimulates the biological N-fixation in soils. This study strongly supports the future application of SRB for the bioremediation of heavy metal-polluted sites.

基金：

Project of the National Natural Science Foundation of China [42230716]; National Major Research and Development Program of China [2023YFC3707600, 2023YFC3207300]; The 111 Project [B21017]; The 1000-Talents Plan Project [WQ2017110423]

语种：

英文

DOI：

10.1016/j.scitotenv.2024.174402

来源：

SCIENCE OF THE TOTAL ENVIRONMENT,2024():.

出版日期：

2024-10-10

提交日期：

2024-08-09

引用参考：

Liu, Houquan; Yao, Jun; Shi, Chunyu; Duran, Robert; Liu, Jianli; Jiang, Shun; Li, Miaomiao; Pang, Wancheng; Ma, Bo; Cao, Ying; Sunahara, Geoffrey. Sulfate-reducing consortium HQ23 stabilizes metal(loid)s and activates biological N-fixation in mixed heavy metal-contaminated soil[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2024():.